Eva Bublova comes from a musical family. At the Prague Conservatory she studied organ and piano, and she earned a degree in organ at the Academy of Performing Arts in Prague under the tutelage of Associate Professor Jaroslav Tuma (2000). Thereafter she studied with Jean Boyer at the Conservatoire National Supérieur de Musique in Lyon, France (2000–2003). Upon returning, she furthered her studies at the Academy of Performing Arts in Prague, this time in the harpsichord class of Giedre Luksaite-Mrazkova (2004–2007).

She has participated in many master classes led by such renowned artists as L. Ghielmi, J. C. Zehnder, M. Sander, L. Lohmann, E. Kooiman, M. Bouvard, O. Latry.

Eva Bublova has been a prizewinner at several international competitions:

  • Prague Spring International Organ Competition 1999
  • Gottfried Silbermann Internazionaler Orgelwettbewerb 2001 in Freiberg (Germany)
  • Johann Joseph Fux Orgelwettbewerb 2005 in Anger (Austria)
  • Concorso Organistico Internazionale "Francesco D'Onofrio" 2006 in Carunchio (Italy)
  • Prix Joseph Bossard 2009 in Bellelay (Switzerland)

In 2002 she was a finalist at the Royal Bank Calgary International Organ Festival and Competition.

As a soloist she has performed with many ensembles (including Tiburtina Ensemble, Musica Bohemica, Harmonie Rudolfinum and Prague Symphony Orchestra FOK), she has made several recordings for Czech, Slovak, Austrian and Luxembourg radio, and she has given concerts in most European countries, Japan and Canada.

In 2005 she released a CD of organ music titled Les Fresques with compositions by K. Slavicky, B. A. Wiedermann, C. Franck and Ch. M. Widor.

She taught organ at Ceske Budejovice Conservatory, at Teplice Conservatory and at the Music School of the City of Prague. She was the organist for the French-speaking Catholic Church in Prague and at the Church of the Holiest Saviour in Prague's Klementinum. Since 2021 she has been teaching organ at Prague Conservatory.